Tee ke e 'ngoe ea lino tse tharo tse kholo tsa lefats'e, tse nang le polyphenols tse ngata, tse nang le antioxidant, anti-cancer, anti-virus, hypoglycemic, hypolipidemic le mesebetsi e meng ea likokoana-hloko le mesebetsi ea bophelo bo botle. Tee e ka aroloa ka tee e sa lomosoang, tee e lomositsoeng le tee e lomositsoeng ho latela theknoloji ea eona ea ho e lokisa le tekanyo ea ho belisoa. Tee e entsoeng ka mor'a ho belisoa e bolela tee e nang le karolo ea likokoana-hloko ho belisoa, joalo ka tee e phehiloeng ea Pu 'er, tee ea Fu Brick, tee ea Liubao e hlahisoang Chaena, le Kippukucha, Saryusoso, Yamabukinadeshiko, Suraribijin le Kuroyamecha e hlahisoang Japane. Li-tea tsena tse belisoang ke likokoana-hloko li ratoa ke batho ka lebaka la litlamorao tsa bona tsa bophelo bo botle joalo ka ho theola mafura a mali, tsoekere e maling le k'holeseterole.

Kamora ho belisoa ha likokoana-hloko, tee ea polyphenols ka tee e fetoloa ke li-enzyme 'me li-polyphenol tse ngata tse nang le meaho e mecha lia thehoa. Teadenol A le Teadenol B ke lintho tse nkiloeng ho polyphenol tse arohaneng le tee e lomositsoeng ka Aspergillus sp (PK-1, FARM AP-21280). Phuputsong e ileng ea latela, e ile ea fumanoa ka bongata ba tee e lomositsoeng. Teadenols e na le li-stereoisomers tse peli, cis-Teadenol A le trans-Teadenol B. Foromo ea molek'hule C14H12O6, boima ba molek'hule 276.06, [MH] -275.0562, mokhoa oa sebopeho o bontšoa ho Setšoantšo sa 1. Li-teadenols li na le lihlopha tsa li-cyclic le tse tšoanang le tsa C- a lesale meaho ea flavane 3-joala le Ke lintho tse nkiloeng karateng ea b-ring fission. Teadenol A le Teadenol B li ka etsoa biosynthesized ho tsoa EGCG le GCG ka ho latellana.

Liphuputsong tse latelang, ho ile ha fumanoa hore Teadenols e na le mesebetsi ea likokoana-hloko e kang ho khothalletsa secretion ea adiponectin, ho thibela polelo ea protheine tyrosine phosphatase 1B (PTP1B) le ho soeufala, e leng se ileng sa hohela tlhokomelo ea bafuputsi ba bangata. Adiponectin ke polypeptide e khethehileng haholo ho lisele tsa adipose, e ka fokotsang haholo ts'oaetso ea mathata a metabolic mofuteng oa II oa lefu la tsoekere. Hona joale PTP1B e nkoa e le sepheo sa phekolo bakeng sa lefu la tsoekere le botenya, e leng se bontšang hore Teadenols e na le liphello tse ka bang teng tsa hypoglycemic le tahlehelo ea boima ba 'mele.

Ka pampiri ena, ho ile ha shejoa tlhahlobo ea litaba, biosynthesis, motsoako o felletseng le bioactivity ea Teadenols ka tee e lomositsoeng ea likokoana-hloko, e le ho fana ka motheo oa saense le tšupiso ea khopolo-taba bakeng sa nts'etsopele le ts'ebeliso ea Teadenols.

▲ TA setšoantšo sa 'mele

01

Ho fumanoa ha li-teadenols ka tee e belisoang ke likokoana-hloko

Kamora hore li-teadenols li fumanoe ho tsoa ho Aspergillus SP (PK-1, FARM AP-21280) khetlo la pele, ho ile ha sebelisoa mekhoa ea HPLC le LC-MS/MS ho ithuta Teadenols ka mefuta e fapaneng ea tee. Boithuto bo bonts'itse hore Teadenols e fumaneha haholo teeng e belisoang ke likokoana-hloko.

▲ TA, chromatogram ea mokelikeli oa lefuba

▲ Ponahalo ea 'mele ea tee e belisoang ke likokoana-hloko le TA le lefuba

Aspergillus oryzae SP.PK-1, FARM AP-21280, Aspergillus oryzae sp.AO-1, ​​NBRS 4214, Aspergillus awamori sp.SK-1, Aspergillus oryzae Sp.AO-1, ​​NBRS 4214, Aspergillus oryzae SK-1 , NBRS 4122), Eurotium sp. Ka-1, FARM AP-21291, likarolo tse fapaneng tsa Teadenols li fumanoe teeng e lomositsoeng ea Kippukucha, Saryusoso, Yamabukinadeshiko, Suraribijin le Kuroyamecha, gentoku-cha e rekisoang Japane, le tee e phehiloeng ea Pu erh, tee ea Liuckbao le Fu Brick. tee ea China.

Likahare tsa Teadenols ka li-tea tse fapaneng li fapane, ho nahanoang hore li bakoa ke maemo a fapaneng a ts'ebetso le maemo a ho belisoa.

Liphuputso tse ling li bonts'itse hore litaba tsa Teadenols makhasi a tee ntle le ts'ebetso ea ho belisoa ha likokoana-hloko, joalo ka tee e tala, tee e ntšo, tee ea oolong le tee e tšoeu, li ne li le tlase haholo, ha e le hantle li le ka tlase ho moeli oa ho fumanoa. Likahare tsa teadenol makhasi a fapaneng a tee li bonts'oa ho Lethathamo la 1.

02

Bioactivity ea Teadenols

Liphuputso li bontšitse hore Teadenols e ka khothalletsa ho theola boima ba 'mele, ho loantša lefu la tsoekere, ho loantša oxidation, ho thibela ho ata ha lisele tsa mofetše le ho soeufatsa letlalo.

Teadenol A e ka khothaletsa secretion ea adiponectin. Adiponectin ke endo native peptide e patiloeng ke adipocyte mme e ikhethang haholo ho lisele tsa adipose. E na le kamano e mpe haholo le lisele tsa visceral adipose mme e na le thepa ea anti-inflammatory le anti-atherosclerotic. Kahoo Teadenol A e na le monyetla oa ho theola boima ba 'mele.

Teadenol A e boetse e thibela polelo ea protheine tyrosine phosphatase 1B (PTP1B), A classic non-receptor tyrosine phosphatase lelapeng la protheine tyrosine phosphatase, e nang le karolo e mpe ea bohlokoa ho bonts'a insulin mme hajoale e amoheloa e le Sepheo sa kalafo bakeng sa lefu la tsoekere. Teadenol A e ka laola insulin hantle ka ho thibela polelo ea PTP1B. Ho sa le joalo, TOMOTAKA et al. e bontšitse hore Teadenol A ke A ligand ea nako e telele fatty acid receptor GPR120, e ka tlamang ka ho toba le ho kenya tšebetsong GPR120 le ho khothalletsa secretion ea insulin hormone GLP-1 ka intestinal endocrine STC-1 liseleng. Glp-1 e thibela takatso ea lijo mme e eketsa secretion ea insulin, e bonts'a litlamorao tse khahlanong le lefu la tsoekere. Ka hona, Teadenol A e na le phello e ka bang teng ea antidiabetic.

Litekanyetso tsa IC50 tsa ts'ebetso ea ho senya DPPH le ts'ebetso ea superoxide anion radical scavenging ea Teadenol A e ne e le 64.8 μg / mL le 3.335 mg / mL, ka ho latellana. Litekanyetso tsa IC50 tsa kakaretso ea matla a antioxidant le matla a phepelo ea haedrojene e ne e le 17.6 U/mL le 12 U/mL, ka ho latellana. Ho boetse ho bonts'itsoe hore tee ea tee e nang le Teadenol B e na le ts'ebetso e phahameng ea anti-proliferating khahlano le lisele tsa mofets'e oa HT-29, mme e thibela lisele tsa mofets'e oa HT-29 ka ho eketsa maemo a polelo ea caspase-3/7, caspase-8 le Caspase. -9, lefu la receptor le litsela tsa apoptosis tsa mitochondrial.

Ntle le moo, Teadenols ke sehlopha sa polyphenols se ka soeufatsang letlalo ka ho thibela tšebetso ea melanocyte le synthesis ea melanin.

03

Motsoako oa Teadenols

Joalo ka ha ho ka bonoa ho data ea lipatlisiso ho Lethathamo la 1, li-teadenols ka tee ea beliso ea likokoana-hloko li na le litaba tse tlase le litšenyehelo tse phahameng tsa ho ntlafatsa le tlhoekiso, e leng ho leng thata ho fihlela litlhoko tsa lipatlisiso tse tebileng le nts'etsopele ea ts'ebeliso. Ka hona, litsebi li entse liphuputso mabapi le ho kopanngoa ha lintho tse joalo ho tloha mahlakoreng a mabeli a biotransformation le lik'hemik'hale tsa lik'hemik'hale.

WULANDARI et al. inoculated Aspergillus SP (PK-1, FARM AP-21280) ka tharollo e tsoakiloeng ea sterilized EGCG le GCG. Kamora libeke tse 2 tsa setso ho 25 ℃, HPLC e ile ea sebelisoa ho sekaseka sebopeho sa setso sa setso. Teadenol A le Teadenol B li fumanoe. Hamorao, Aspergillus oryzae A. Awamori (NRIB-2061) le Aspergillus oryzae A. Kawachii (IFO-4308) ba ile ba kenngoa ho A motsoako oa autoclave EGCG le GCG, ka ho latellana, ho sebelisa mokhoa o tšoanang. Teadenol A le Teadenol B li fumanoe ka bobeli. Liphuputso tsena li bontšitse hore phetoho ea likokoana-hloko ea EGCG le GCG e ka hlahisa Teadenol A le Teadenol B. SONG et al. e sebelisitse EGCG e le lisebelisoa tse tala le ente ea Aspergillus sp ho ithuta maemo a nepahetseng bakeng sa tlhahiso ea Teadenol A le Teadenol B ka setso sa metsi le se tiileng. Liphetho li bonts'itse hore mokhoa o fetotsoeng oa CZapEK-DOX o nang le 5% EGCG le 1% phofo ea tee e tala e na le chai e ngata. Ho ile ha fumanoa hore tlatsetso ea phofo ea tee e tala ha ea ka ea ama ka ho toba tlhahiso ea Teadenol A le Teadenol B, empa haholo-holo e ile ea etsa hore ho be le keketseho ea palo ea biosynthase e amehang. Ho phaella moo, YOSHIDA et al. e entsoeng Teadenol A le Teadenol B ho tloha phloroglucinol. Mehato ea bohlokoa ea ho kopanya e ne e le asymmetric α -aminoxy catalytic reaction of organic catalytic aldehydes le intramolecular allyl substitution ea palladium-catalyzed phenol.

▲ Microscopy ea elektrone ea ts'ebetso ea ho belisoa ha tee

04

Boithuto ba tšebeliso ea Teadenols

Ka lebaka la ts'ebetso ea eona ea bohlokoa ea likokoana-hloko, li-Teadenols li 'nile tsa sebelisoa ho etsa meriana, lijo le lijo, litlolo, li-reagents tsa ho lemoha le masimo a mang.

Ho na le lihlahisoa tse amanang le li-Teadenols tšimong ea lijo, joalo ka Tea ea Slimming ea Japane le li-polyphenols tsa tee tse lomositsoeng. Ho phaella moo, Yanagida et al. e netefalitse hore li-extracts tsa tee tse nang le Teadenol A le Teadenol B li ka sebelisoa ho lokisa lijo, li-condiments, li-supplement tsa bophelo bo botle, lijo tsa liphoofolo le litlolo. ITO le ba bang. e lokiselitse sesebelisoa sa letlalo se nang le li-teadenols tse nang le phello e matla ea bosoeu, inhibition ea mahala ea radical le anti-wrinkle effect. E boetse e na le litlamorao tsa ho phekola makhopho, ho nosetsa, ho matlafatsa ts'ebetso ea thibelo, ho thibela ho ruruha ho tsoang ho UV le liso tse khahlanong le khatello ea maikutlo.

Chaena, Teadenols e bitsoa fu tee. Bafuputsi ba entse liphuputso tse ngata ka li-extracts tsa tee kapa liforomo tsa motsoako tse nang le fu tea A le Fu tee ea B mabapi le ho theola lipids maling, ho theola boima ba 'mele, tsoekere ea mali, khatello ea mali le ho nolofatsa methapo ea mali. Tee ea fu e phahameng e hloekisitsoeng le ho lokisoa ke Zhao Ming et al. E ka sebelisoa bakeng sa ho lokisa litlhare tsa antilipid. He Zhihong et al. e entse li-capsules tsa tee, matlapa kapa li-granules tse nang le tee e lefifi ea anhua ea Fu A le Fu B, gynostema pentaphylla, Rhizoma orientalis, ophiopogon le lihlahisoa tse ling tsa meriana le tsa lijo, tse nang le litlamorao tse hlakileng le tse tšoarellang ho theoheng ha boima ba 'mele le phokotso ea lipid bakeng sa mefuta eohle ea botenya. batho. Tan Xiao 'ao o lokiselitse tee ea fuzhuan ka fuzhuan A le Fuzhuan B, e bonolo ho monngoa ke' mele oa motho 'me e na le liphello tse hlakileng ho fokotsa hyperlipidemia, hyperglycemia, khatello ea mali le ho nolofatsa methapo ea mali.

05

“Puo

Li-teadenols ke li-b-ring fission catechin derivatives tse teng teeng e belisoang ea likokoana-hloko, tse ka fumanoang phetohong ea likokoana-hloko tsa epigallocatechin gallate kapa ho tsoa ho kakaretso ea phloroglucinol. Boithuto bo bonts'itse hore li-teadenols li fumaneha ka har'a li-teas tse fapaneng tse belisoang ke likokoana-hloko. Lihlahisoa li kenyelletsa tee e lomositsoeng ea Aspergillus Niger, Aspergillus oryzae ferment tea, Aspergillus oryzae ferment tea, Sachinella fermented tea, Kippukucha (Japan), Saryusoso (Japan), Yamabukinadeshiko (Japan), Suraribijin (Japan), Kuroyamecha (Japan), Gentok U-Japan), cha (Japan), Awa-Bancha (Japan), Goishi-cha (Japan), tee ea Pu 'er, tee ea Liubao le tee ea Fu Brick, empa likahare tsa Teadenols ka litee tse fapaneng li fapane haholo. Likahare tsa Teadenol A le B li ne li tloha ho 0.01% ho ea ho 6.98% le 0.01% ho ea ho 0.54%, ka ho latellana. Ka nako e ts'oanang, li-oolong, tse tšoeu, tse tala le tse ntšo ha li na metsoako ena.

Ho ea ka lipatlisiso tsa morao-rao, lithuto tsa Teadenols li ntse li lekanyelitsoe, tse kenyelletsang mohloli feela, likahare, biosynthesis le tsela e feletseng ea maiketsetso, le mokhoa oa eona oa ts'ebetso le nts'etsopele le ts'ebeliso e ntse e hloka lipatlisiso tse ngata. Ka lipatlisiso tse ling, metsoako ea Teadenols e tla ba le boleng bo boholo ba nts'etsopele le tebello e pharaletseng ea ts'ebeliso.