Shaadu waa mid ka mid ah saddexda cabitaan ee adduunka, oo hodan ku ah polyphenols, oo leh antioxidant, kansarka, fayraska, hypolitemiction, hypolitemic, nashaadaadyo bayooloji ah iyo howlaha daryeelka caafimaadka iyo howlaha daryeelka caafimaadka. Shaaha waxaa loo qaybin karaa shaaha aan la koobi karin, shaaha la kariyey iyo shaaha ka dib markii loo yaqaan tikniyoolajiyadda howsha iyo heerka halsano. Shaah ka dib ayaa waxaa loola jeedaa shaah iyadoo kaqaybgalka microfiya ee halsano, shaaha shaabaabka la kariyey, shaauhu, ee Kuribiyin, YAMBUINDESHO, YAMABIYAY iyo KURYOYEHESENED ee ka soo saaray Japan. Shaahani mikraka ah ee mikraka ah ee microbial-ka ah waxaa jecel dadka ku saabsan saameynta daryeelka caafimaad sida hoos u dhigista baruurta dhiigga, sonkorta dhiigga iyo kolestaroolka dhiigga.

Ka dib markii halsano microbial ka dib, polyphenols shaaha ee shaah ah waxaa beddelaya enzymes-ka iyo polyphenol badan oo leh qaab-dhismeedka cusub ayaa la sameeyay. TEDENENOL A iyo TEDENOL B Ma yihiin kuwo soo saaray polyphenol oo go'doonsan oo ka soo baxa shaaha ka jilicsan oo ay la socdaan Askphilles SP (PK-1, beerta AP-21280). Daraasad ku xigta, waxaa lagu ogaaday tiro aad u badan oo shaah ah oo la kariyey. TEWenols waxay leeyihiin labo iskood umeer, CIS-TEDENenol B. Qaaciddada Molecaular C14h12o6, [MH12O6, [MH12O6) 27.06. Catchelins-ka Cathechins. TEDENENENENENOL A iyo TEDenol B ayaa laga yaabaa inuu bioseysiyaa laga helo EGCG iyo GCG siday u kala horreeyaan.

Daraasadaha ku xiga, waxaa la ogaaday in tareenka la sameeyay ay yeesheen howlo bayooloji ah sida dhiirrigelinta dheecaannada adipositin, oo ka horjoogsanaya protein anyphaasase 1B (PTP1B) (PTP1B) Muujinta iyo caddeynta, taas oo soo jiidatay dareenka cilmi-baarayaal badan. Adiponictive waa polypeptide-ka si aad u gaar ah oo loo yaqaan 'polype polypheptide', kaas oo si weyn hoos ugu dhigi kara dhacdooyinka ciladaha dheef-shiid kiimikaadka ee nooca sonkorowga nooca II. PTP1b waxaa hada loo aqoonsan yahay inuu yahay bartilmaameedka daaweynta ee sonkorowga iyo buurnaanta, taasoo muujineysa in teadenols ay leedahay hypoglycemic-ka ay suuragal tahay iyo culayska miisaan luminta.

Warqaddan, ogaanshaha waxyaabaha ku jira, bisyntsis, wadarta synthesis-ka iyo biosetesis-ka iyo biosenesity of teadenols ee shaah microbial-ka ah ayaa dib loo eegay, si loo tixraacayo saboolnimada iyo tixraaca aragtida horumarka iyo ka-faa'iideysiga tareenka.

▲ Sawirka jirka

01

Ogaanshaha TEADENOLS ee shaah miraha ah ee microbial

Ka dib markii la soo saaray ka dib markii laga soo qaatay Askphilles SP (PK-1, Beer AP-21280) TEA-yada ugu horreeya, HPLC iyo Ms / Ms / Ms / Ms / Ms / Ms / Ms / Ms / Ms / Ms / Ms / MS. Daraasaduhu waxay muujiyeen in tineduls inta badan ay ka jiraan shaah microbial-ka microbial.

▲ Ta, TB dareere dareere ah

▲ Mass Spectrometry oo ah shaah mikreysan oo microbial ah iyo TA iyo tiibayda

Aspergillus Oryzae SP.pk-1, Beer AP-21280, ASPERTGILL SP.Ao-1, NBRS-1, NBRS-1, NBRS-1, NBRS - NBRS - NBRS 4122), Eurotium 4122), Eurotium sp. Ka-1, Beer AP-21291, uruurin kala duwan oo ka mid ah TEWUSCOLs, ee Sareonaladeshiko, Suriona-ka, iyo Kuryokicha, oo ah shaah ee lagu kariyey Pum, LiaBao Shaaha iyo shaah ee Firl.

Waxyaabaha ay ka kooban yihiin teadnols ee shaah kala duwan ayaa ka duwan, kaas oo loo yaqaan in ay sababaan xaaladaha kala duwan ee howsha iyo xaaladaha halsano.

Daraasado dheeri ah ayaa muujisay in nuxurka tareenka ee caleemaha shaaha iyada oo aan la helin halsano microbial, sida shaaha cagaaran, shaaha cagaaran, iyo shaah cad, asal ahaan ka hooseeya xadka ogaanshaha. TEXENENENENENENENOL EE GUUD EE CUSUB EE SHARADA SHARABARKA ayaa lagu muujiyey shaxda 1.

02

Bioccactivity of teadenols

Daraasaduhu waxay muujiyeen in tineduenol ay kor u qaadi karaan miisaanka oo yaraada, la dagaalanka sonkorowga, la-dagaallanka digidation, oo xakameynaya faafitaanka unugyada kansarka iyo maqaarka whiten.

TEDenol A ayaa horumarin kara dheecaanka adiposickinectin. Adiponictkin waa peptous peptide pettide oo ay qarsoon tahay adipocytes iyo si aad u gaar ah unugyada adipsese. Aad ayey ula xiriirtaa unugyada visceral adi edipose kuna leh anti-bararka iyo guryaha anti-atherosclerotic-ka. Marka TEDenol A ayaa leh awood ay ku lumiso miisaanka.

TEDENENENENENENENENENENENENENENENENENENENENENENENENENENENENENENENE AYAAN KA QAYB QAADASHADA TIRONINE THEROSINE PROSPHATEESPHASPHE 1B (PTP1B), oo ah door aan caadi ahayn oo ka mid ah caqabadda insulinta, oo hadda loo aqoonsanayo bartilmaameedka sonkorowga. TEDENENENENENENOL AAD UGU SAMEEYSAA INUU KU SAMEEYO INSULIN OO KU SAABSAN MACLUUMAADKA PTP1B. Dhanka kale, Tomotaka et al. Waxay muujisay in TENENOL AANDAND CRANDE CHAND 'FASTTOR GPROTOR GPR120, kaas oo si toos ah u xiri kara oo uu kor u qaadi karaa GPR120 oo kor u qaadi kara dheecaanka hormoonka insulin-ka-1 ee unugyada encestinne-ka. GLP-1 wuxuu xannibayaa rabitaanka cuntada wuxuuna kordhiyaa sirta insulinta, oo muujinaya saameyn-ka-hortagga sonkorowga. Sidaa darteed, TEDENOL A waa saameyn suurto gal ah oo ka hortaga daawada ka hortagga.

Qiimaha IC50 ee ah qarashka kala-sooca waxqabadka iyo aqalka sare ee sabreenka leh dhaqdhaqaaqa qalloocinta ee TEDENenol a waxay ahayd 64.8 μg / ml iyo 3.335 mg / ml, siday u kala horreeyaan. Qiimaha IC50 ee wadarta awoodda Antioxidant iyo awooda sahayda hydrogen waxay ahaayeen 17.6 u / ml iyo 12 u / ml, siday u kala horreeyaan. Waxaa sidoo kale la muujiyay in soosaarka shaaha ee ay ka kooban tahay TEENENENENENOL B ee loo yaqaan 'TENPENOL B'

Intaas waxaa sii dheer, tinevanols waa fasal ku yaal polyphenols oo cadday kara maqaarka iyagoo joojineysa nashaada melanocyte iyo melanin synthesis.

03

Isku-darka tineduls

Sida laga arki karo xogta cilmi baarista ee Shaaha 1, TEWenols ee shaahka halsano microbial-ka microbial-ka microbial-ka ayaa leh waxyaabo hooseeya iyo qiimo sare oo kobcinta iyo nadiifinta, taas oo ay adag tahay in la daboolo baahiyaha cilmi-baarista qoto dheer iyo horumarka dalabka. Sidaa darteed, culimadu waxay ku sameeyeen daraasado ku saabsan isku-darka walxahaas oo ka kooban laba jiho oo ah biotrasismentation iyo synthesis kiimikada.

Wunstari et al. Waxaa loo ixtiraamay Aspergillus SP (PK-1, Beer AP-21280) ee xalka isku dhafan ee jeermisku iyo GCG. Ka dib 2 toddobaad oo Dhaqanka ah ee 25 ℃, HPLC waxaa loo isticmaalay in lagu falanqeeyo qaabka dhexdhexaadka dhaqanka. TEDENOL A iyo TEDenol b ayaa la ogaaday. Goor dambe, ASPERGILLUE Oryzae A. Awamori (Nrib-2061) iyo Asperdillus Oyzae A. Kawachi (IFO-4308) waxaa lagu dhexjiray isku dar ah Esclave iyo GCG, oo loo kala horreeyo isla habkaas. TEDENOL A iyo TEDenol b ayaa lagu ogaadey in labadaba dhexdhexaadnimo. Daraasadahaani waxay muujiyeen in isbadalka microbial ee EGCG iyo GCG ay soo saari karaan TEDENOL A iyo TENENOL B. Song Et Al. Waxaa loo adeegsaday EGCG sida ashyaa'da ceyriinka ah iyo astauculated Aspergillus sp si ay u bartaan xaaladaha ugu habboon ee wax soo saarka timaha iyo wax soo saarka tinenenol b oo dareere ah iyo dhaqan adag. Natiijooyinka waxay muujiyeen in kuwa wax laga beddelay ee ku jira dhexdhexaadka Csapek-dox ee ku jira 5% EGCG iyo 1% budada shaaha cagaaran ayaa leh dhalidda ugu sareysa. Waxaa la ogaaday in lagu daro ee budada shaaha cagaaran aysan si toos ah saameyn ugu lahayn soosaarka TEDENENOL A iyo TEDenol B, laakiin inta badan waxay kordhisay kororka tirada bisyntre-ka ku lug leh. Intaa waxaa sii dheer, Yoshida et al. TEDENENENESED TEDENENENENOL A iyo TEDENOL B oo ka socota Phloroglucinol. Tallaabooyinka ugu muhiimsan ee synthesis-ka waxay ahaayeen asymmetric ααaminaxy-ka falcelinta udhaca ee 'alehydes' iyo ka beddelka 'Intramolecular Alll' ee palladium-ka-soo-nooleynta palladium.

▲ Electron elektaroonigga ah ee habka loo yaqaan 'The Traint'

04

Daraasadda Codsiga ee TEWenols

Sababo la xiriira firfircoonideeda noolaha ee muhiimka ah, tareenka waxaa loo isticmaalay dawooyinka, cuntada iyo quudinta, waxyaabaha la isku qurxiyo, dib-u-soo-nooleynta iyo meelaha kale.

Waxaa jira alaabooyin la xiriira oo ay ku jiraan tabolenol ku yaal garoonka cuntada, sida shaaha japanese shaaha japanese iyo polyphenols shaaha oo kacsan. Intaas waxaa sii dheer, Yanagida et al. Xaqiijiyay in soosaarka shaaha ee ay ku jiraan TEDENenol A iyo TEDenol B waxaa lagu dabaqi karaa howsha cuntada, xambaarrada, quudinta, quudinta xoolaha iyo waxyaabaha la isku qurxiyo. ITO et al. Waxaa loo diyaariyey wakiilka maqaarka ah ee maqaarka ah oo ay kujiraan tareenka ku jira saameyn adag oo caddaalad ah, oo ah xannibaadda xag-jirnimada iyo saamaynta ka hortagga duuduubka. Waxay kaloo leedahay saamaynta daweynta finanka, qoyaanka qoyaanka, kor u qaadida shaqada caqabadda, oo ka horjoogsanaysa caabuqa ummada iyo nabaro ka hortagga cadaadiska.

Shiinaha gudaheeda, tieunols waxaa loo yaqaannaa shaah FU. Baarayaasha waxay ku sameeyeen waxbarasho badan oo ku saabsan soosaarka shaaha ama qaacidooyinka isku-darka shaaha ama qaacidooyinka isku-darka ah ee ay ka kooban yihiin hoos u dhigista hoos u dhigista libaaxyada dhiigga, sonkorta dhiigga, dhiig karka, dhiig karka iyo jilcida xididdada dhiigga. Shaah sare ee daahirnimada ah ee la nadiifiyey oo ay diyaariyeen Zhao Ming et al. Waxaa loo isticmaali karaa diyaarinta dawooyinka ka hortagga kantaristipid. Isagu zhihong et al. Waxay ka dhigtay shaaha shaaha, kiniiniyada ama granua-ka oo ay kujiraan shaah mugdi ah oo ah gu b, gyizomation-ka, oo leh saameynno isdaba joog ah iyo hoos u dhac ku saabsan yareynta miisaanka iyo yareynta caynkaabka dadka oo dhan ee dadka qurxinta. TANO 'AO waxay u diyaarisay shaaha Fuzhuan oo leh Fuzhuan a iyo Fuzhuan B, kaas oo fududahay in la nuugo jirka oo uu leeyahay saameyn yar oo ku saabsan yareynta hyperllipidemia, hyperglycemia, dhiig karka iyo jilcida xididdada dhiigga.

05

Luuqad

TEDENOL-yada ayaa ah kuwa galka galka ah ee BRAING CATECHING Ruthing oo ka jira shaaha michoolobial, oo laga heli karo isbadalka microbial ee epigallocathinglatechin ama wadarta isku-darka 'phloroglucinol'. Daraasaduhu waxay muujiyeen in tinedulol-ka ay kujiraan shaashado kala duwan oo mikreysan. Alaabada waxaa ka mid ah Aspergillus shaah shaaha ah, ASPERGILLUS Oryzae TEA, shaatirikar, Sachisichadeshiko (Japan), Kuryoyamca (Japan), Kurrok U-Char (Japan), Awa-Banchsha (Japan), Gooshiis-Cha (Japan), PU TAE, Lia-Cha (Japan), PU Shaate, Lia-Cha (Japan), PU Shaate, Lia-Cha Waxyaabaha ay ka kooban tahay TEDENOL A iyo B waxay ka soo baxaan 0.01% ilaa 6.98% iyo 0.01% ilaa 0.54%, siday u kala horreeyaan. Isla mar ahaantaana, fiilong, cad, cagaar iyo madow oo madow ah kuma jiraan xeryahan.

Ilaa iyo inta cilmi baarista ee hadda jirta ay ka walaacsan tahay, daraasadaha lagu sameeyo tinexantu wali waa mid xadidan, oo ku lug leh kaliya isha, waxa ku jira, biosynthesis-ka iyo guud ahaan dariiqa waxqabadka iyo horumarka iyo arjiga iyo codsigu wali waxay u baahan yihiin cilmi-baaris badan. Iyada oo leh cilmi-baaris dheeraad ah, xeryahooda tibenaduhu waxay yeelan doonaan qiime sare oo horumar leh iyo rajo ballaaran oo codsi ah.