O le lauti o se tasi lea o meainu tetele e tolu a le lalolagi, e mauoa i polyphenols, e iai le antioxidant, anti-cancer, anti-virus, hypoglycemic, hypolipidemic ma isi gaioiga faʻaola ma galuega tau soifua maloloina. E mafai ona vaevaeina lauti i lauti e le fa'afefeteina, lauti fa'afefeteina ma lauti fa'avela e tusa ai ma lona gaosiga tekinolosi ma le tikeri o le fa'afefeteina. O lauti Post-fermented e faatatau i lauti ma microbial auai i fermentation, e pei o le lauti kuka Pu 'er, Fu Brick lauti, Liubao lauti gaosia i Saina, Ma Kippukucha, Saryusoso, Yamabukinadeshiko, Suraribijin ma Kuroyamecha gaosia i Iapani. O nei lauti fa'afefete microbial e alofagia e tagata ona o latou a'afiaga fa'alesoifua maloloina e pei o le fa'aitiitia o ga'o toto, suka toto ma le cholesterol.

A maeʻa le faʻafefeteina o microbial, o le lauti polyphenols i lauti e liua e enzymes ma tele polyphenols ma fausaga fou ua fausia. Teadenol A ma Teadenol B o polyphenol e maua mai i lauti fa'afefete ma Aspergillus sp (PK-1, FARM AP-21280). I se suʻesuʻega mulimuli ane, na maua i le tele o lauti faʻafefe. Teadenols e lua stereoisomers, cis-Teadenol A ma trans-Teadenol B. Molecular formula C14H12O6, mole mamafa 276.06, [MH] -275.0562, fa'atusa fa'atulagaina o lo'o fa'aalia i le Ata 1. Teadenols ei ai vaega ta'amilosaga tutusa ma le mama ma le C- fausaga mama o le flavane 3-alcohols ma o le b-mama fission catechins derivatives. Teadenol A ma Teadenol B e mafai ona biosynthesized mai le EGCG ma le GCG.

I suʻesuʻega mulimuli ane, na maua ai le Teadenols o loʻo i ai ni gaioiga faʻapitoa e pei o le faʻalauiloaina o le adiponectin secretion, faʻalavelaveina le faʻaaliga o le tyrosine phosphatase 1B (PTP1B) ma le paʻepaʻe, lea na tosina mai ai le tele o tagata suʻesuʻe. Adiponectin o se polypeptide sili ona patino i le tino, lea e mafai ona faʻaitiitia ai le aʻafiaga o faʻamaʻi pipisi i le maʻisuka ituaiga II. O le PTP1B o lo'o amana'ia i le taimi nei o se togafiti fa'apitoa mo le ma'i suka ma le tino puta, e ta'u mai ai o Teadenols e ono a'afia ai le hypoglycemic ma le pa'u mamafa.

I totonu o lenei pepa, o le suʻesuʻeina o mea, biosynthesis, faʻasologa atoa ma le bioactivity o Teadenols i le lauti faʻafefeteina microbial na toe iloiloina, ina ia maua ai le faʻasaienisi ma faʻamatalaga faʻamatalaga mo le atinaʻeina ma le faʻaaogaina o Teadenols.

▲ TA ata faaletino

01

Su'esu'eina o Teadenols i le lauti fa'afefeteina microbial

Ina ua maua mai Teadenols mai Aspergillus SP (PK-1, FARM AP-21280) lauti fa'afefeteina mo le taimi muamua, sa fa'aogaina auala HPLC ma LC-MS/MS e su'esu'e ai Teadenols i ituaiga lauti eseese. O su'esu'ega ua fa'aalia ai o Teadenols e tele lava ina maua i lauti fa'afefeteina.

▲ TA, TB vai chromatogram

▲ Mass spectrometry o lauti faafefete microbial ma TA ma TB

Aspergillus oryzae SP.PK-1, FARM AP-21280, Aspergillus oryzae sp.AO-1, ​​NBRS 4214, Aspergillus awamori sp.SK-1, Aspergillus oryzae Sp.AO-1, ​​NBRS 4214, Aspergillus oryzae sp.SK-1 , NBRS 4122), Eurotium sp. Ka-1, FARM AP-21291, Eseese concentrations o Teadenols na maua i le lauti faafefete Kippukucha, Saryusoso, Yamabukinadeshiko, Suraribijin ma Kuroyamecha, gentoku-cha faatau atu i Iapani, ma i le lauti kuka o Pu erh, Liubao lauti ma Fu Brick lauti a Saina.

O mea o loʻo i totonu o Teadenols i lauti eseese e ese, lea e taumatemate e mafua mai i tulaga eseese o gaioiga ma tulaga faʻafefe.

O isi su'esu'ega na fa'aalia ai o mea o lo'o i totonu o Teadenols i lau lauti e aunoa ma le fa'aogaina o le microbial fermentation, e pei o le lauti lanumeamata, lauti uliuli, lauti oolong ma le lauti pa'epa'e, na matua maualalo lava, i lalo ifo o le fa'atapula'aina. O lo'o iai le Teadenol i lau lauti eseese o lo'o fa'aalia i le Laulau 1.

02

Bioactivity o Teadenols

O suʻesuʻega ua faʻaalia ai e mafai e Teadenols ona faʻaleleia le paʻu o le mamafa, tau le maʻisuka, faʻamaʻi faʻamaʻi, taofia le faʻateleina o sela o le kanesa ma paʻepaʻe paʻu.

Teadenol A e mafai ona faʻalauteleina le faʻaogaina o le adiponectin. Adiponectin o se peptide endogenous natia e adipocytes ma sili ona patino i le tino. E matua le lelei le feso'ota'iga ma le visceral adipose tissue ma e iai mea fa'ato'aga ma fa'ama'i-atherosclerotic. O lea la e iai le malosi o Teadenol A e fa'aitiitia ai le mamafa.

O le Teadenol A e taofia foi le faʻaalia o le protein tyrosine phosphatase 1B (PTP1B), O le tyrosine phosphatase e le masani ai i totonu o le aiga o le tyrosine phosphatase protein, lea e iai sona sao taua i le faʻailoga o le inisalini ma o loʻo amanaʻia nei o se taulaʻi togafitiga mo le maʻisuka. Teadenol A e mafai ona faʻatonutonu lelei le inisalini e ala i le faʻalavelaveina o le faʻaaliga PTP1B. I le taimi nei, TOMOTAKA et al. na faʻaalia ai o Teadenol A o se ligand o le gaʻo gaʻo gaʻo umi filifili GPR120, lea e mafai ona fusifusia saʻo ma faʻagaoioia le GPR120 ma faʻamalosia le faʻaogaina o le inisalini hormone GLP-1 i totonu ole intestinal endocrine STC-1 sela. O le Glp-1 e taofia ai le fia'ai ma fa'ateleina le fa'aliloina o le inisalini, e fa'aalia ai a'afiaga o le ma'i suka. O le mea lea, o Teadenol A e iai se a'afiaga e fa'ama'i suka.

O le IC50 tau o le DPPH scavenging activity ma superoxide anion radical scavenging activity of Teadenol A o 64.8 μg / mL ma 3.335 mg / mL, faasologa. O le tau o le IC50 o le malosi atoa o le antioxidant ma le hydrogen sapalai o le 17.6 U/mL ma le 12 U/mL. Ua fa'aalia fo'i o le vaega o lauti o lo'o i ai Teadenol B e maualuga lona fa'ato'aga e fa'asaga i sela o le kanesa colon HT-29, ma fa'alavelaveina sela o le kanesa colon HT-29 e ala i le fa'ateleina o le fa'aaliga o le caspase-3/7, caspase-8 ma le Caspase. -9, tali maliu ma ala apoptosis mitochondrial.

E le gata i lea, o Teadenols o se vasega o polyphenols e mafai ona faʻamaeʻaina le paʻu e ala i le taofia o le melanocyte gaioiga ma le faʻaogaina o le melanin.

03

Le tuufaatasiga o Teadenols

E pei ona mafai ona vaʻaia mai faʻamaumauga suʻesuʻe i le Laulau 1, Teadenols i microbial fermentation tea e maualalo le anotusi ma le maualuga o le tau o le faʻatamaoaigaina ma le faʻamamāina, lea e faigata ona faʻafetaui manaʻoga o suʻesuʻega loloto ma le atinaʻeina o talosaga. O le mea lea, na faia ai e le au atamamai suʻesuʻega i le tuʻufaʻatasia o ia mea mai itu e lua o le biotransformation ma le vailaʻau faʻasolosolo.

WULANDARI et al. tui Aspergillus SP (PK-1, FARM AP-21280) i le fofo fefiloi o sterilized EGCG ma GCG. Ina ua mae'a le 2 vaiaso ole aganu'u ile 25 ℃, sa fa'aogaina le HPLC e iloilo ai le tu'ufa'atasiga ole aganu'u. Teadenol A ma Teadenol B na maua. Mulimuli ane, o Aspergillus oryzae A. Awamori (NRIB-2061) ma Aspergillus oryzae A. Kawachii (IFO-4308) na faʻapipiʻiina i totonu O se faʻafefiloi o le autoclave EGCG ma le GCG, i le faasologa, e faʻaaoga ai le auala tutusa. Teadenol A ma Teadenol B na maua i auala uma e lua. O nei suʻesuʻega ua faʻaalia ai o le suiga ole microbial ole EGCG ma le GCG e mafai ona maua ai Teadenol A ma Teadenol B. SONG et al. fa'aogaina le EGCG e fai ma mea mata ma tui Aspergillus sp e su'esu'e ai tulaga sili ona lelei mo le gaosiga o Teadenol A ma Teadenol B e ala ile aganu'u vai ma le malosi. O fa'ai'uga na fa'aalia ai o le CZapEK-DOX fa'aliliuina o lo'o iai le 5%EGCG ma le 1% o le pa'u lauti lanumeamata e sili ona maualuga lona fua mai. Na maua o le faʻaopopoina o le pauta lauti lanumeamata e leʻi aʻafia ai le gaosiga o Teadenol A ma Teadenol B, ae faʻateleina le faʻateleina o le aofaʻi o le biosynthase o loʻo aʻafia ai. E le gata i lea, YOSHIDA et al. fa'apipi'iina Teadenol A ma Teadenol B mai le phloroglucinol. O laasaga autu o le faʻapipiʻiina o le asymmetric α -aminoxy catalytic reaction o organic catalytic aldehydes ma intramolecular allyl substitution o palladium-catalyzed phenol.

▲ Electron microscopy o le fa'agasologa o lauti

04

Suʻesuʻega faʻaoga o Teadenols

Ona o lona taua tele o meaola, Teadenols ua faʻaaogaina i vailaʻau, meaʻai ma meaʻai, mea teuteu, mea e suʻesuʻe ai ma isi fanua.

O lo'o iai oloa fa'afeso'ota'i o lo'o iai Teadenols ile fanua taumafa, e pei ole Japanese Slimming Tea ma polyphenols fa'afefete. E le gata i lea, o Yanagida et al. fa'amaonia mai e mafai ona fa'aogaina mea'ai lauti o lo'o iai Teadenol A ma Teadenol B i le fa'agaioiina o mea'ai, mea'ai, mea'ai fa'alesoifua maloloina, mea'ai manu ma mea teuteu. ITO et al. saunia se sui autu o le paʻu o loʻo i ai Teadenols faʻatasi ai ma le malosi paʻepaʻe malosi, faʻalavelave faʻamalosi saoloto ma aʻafiaga e teteʻe maanuminumi. E iai fo'i a'afiaga o le togafitia o le pa'u, fa'asusu, fa'aleleia galuega fa'alava, fa'alavelave le fulafula e maua mai i le uV ma le pa'u o le mamafa.

I Saina, e taʻua Teadenols tea fu. Ua faia e le au su'esu'e le tele o su'esu'ega i le lauti su'esu'e po'o fua fa'afefiloi e iai le fu tea A ma le Fu tea B e fa'atatau i le fa'aitiitia o ga'o toto, pa'u mamafa, suka toto, toto maualuga ma fa'amalulu a'a toto. Le maualuga-mama fu lauti A faamamaina ma saunia e Zhao Ming et al. e mafai ona faʻaaogaina mo le saunia o vailaʻau antilipid. He Zhihong et al. faia o lauti capsules, papa po'o granules o lo'o i ai le lauti uliuli anhua o Fu A ma Fu B, gynostema pentaphylla, Rhizoma orientalis, ophiopogon ma isi vailaʻau ma meaʻai homology oloa, lea ei ai aafiaga manino ma tumau i le paʻu o le mamafa ma le faʻaitiitia o le lipid mo ituaiga uma o gaʻo. tagata. Na saunia e Tan Xiao 'ao le lauti fuzhuan ma fuzhuan A ma Fuzhuan B, lea e faigofie ona mitiia e le tino o le tagata ma ei ai aafiaga manino i le faaitiitia hyperlipidemia, hyperglycemia, toto maualuga ma faamaluluina toto.

05

“Gagana

Teadenols o le b-ring fission catechin derivatives o loʻo i ai i microbial fermented tea, lea e mafai ona maua mai le microbial transformation o le epigallocatechin gallate poʻo le aofaʻi atoa o le phloroglucinol. O suʻesuʻega ua faʻaalia ai o Teadenols o loʻo i totonu o le tele o lauti faʻafefeteina. O oloa e aofia ai le lauti fa'afefete Aspergillus Niger, lauti fa'afefete Aspergillus oryzae, lauti fa'afefete Aspergillus oryzae, lauti fa'afefete Sachinella, Kippukucha (Iapani), Saryusoso (Iapani), Yamabukinadeshiko (Iapani), Suraribijin (Iapani), Kuroyamecha (Iapani), Gentok U. cha (Iapani), Awa-Bancha (Iapani), Goishi-cha (Iapani), Pu'er tea, Liubao tea ma Fu Brick tea, ae o mea o loʻo i totonu o Teadenols i lauti eseese e matua ese lava. O mea o loʻo i totonu o Teadenol A ma le B na amata mai i le 0.01% i le 6.98% ma le 0.01% i le 0.54%, i le faasologa. I le taimi lava e tasi, o le oolong, paʻepaʻe, lanu meamata ma lanu uliuli e le o iai nei mea faʻapipiʻi.

E tusa ai ma suʻesuʻega o loʻo iai nei, o suʻesuʻega i Teadenols e faʻatapulaʻaina, e aofia ai naʻo le puna, anotusi, biosynthesis ma le aofaʻi o auala faʻapipiʻi, ma lona faiga o gaioiga ma atinaʻe ma faʻaoga e manaʻomia pea le tele o suʻesuʻega. Fa'atasi ai ma su'esu'ega fa'aopoopo, o fa'aputuga Teadenols o le a sili atu le tau fa'atupuina ma lautele fa'amoemoega fa'aoga.