Icyayi ni kimwe mu binyobwa bitatu bikomeye ku isi, bikungahaye kuri polifenol, hamwe na antioxydeant, anti-kanseri, anti-virusi, hypoglycemic, hypolipidemic n’ibindi bikorwa by’ibinyabuzima ndetse n’ibikorwa byo kwita ku buzima. Icyayi gishobora kugabanywamo icyayi kidasembuye, icyayi gisembuye hamwe nicyayi nyuma yisembuye ukurikije ikoranabuhanga ryacyo no kurwego rwa fermentation. Icyayi nyuma yo gusembura bivuga icyayi gifite mikorobe igira uruhare mu gusembura, nk'icyayi cyatetse cya Pu 'er, icyayi cya Fu Brick, icyayi cya Liubao gikorerwa mu Bushinwa, Na Kippukucha, Saryusoso, Yamabukinadeshiko, Suraribijin na Kuroyamecha bikorerwa mu Buyapani. Icyayi cya ferment ya mikorobe ikundwa nabantu kubera ingaruka zubuzima bwabo nko kugabanya ibinure byamaraso, isukari yamaraso na cholesterol.

Nyuma yo gusembura mikorobe, icyayi cya polifenole mu cyayi gihindurwa na enzymes kandi hakorwa polifenol nyinshi ifite imiterere mishya. Teadenol A na Teadenol B ni ibikomoka kuri polifenol bitandukanijwe nicyayi gisembuye hamwe na Aspergillus sp (PK-1, FARM AP-21280). Mu bushakashatsi bwakurikiyeho, bwagaragaye mu cyayi cyinshi. Teadenol ifite stereoisomers ebyiri, cis-Teadenol A na trans-Teadenol B. Ifumbire ya molekuline C14H12O6, uburemere bwa molekile 276.06, imiterere yimpeta ya flavane 3-alcool kandi ni b-impeta ya catechins ikomoka. Teadenol A na Teadenol B birashobora kuba biosynthesize muri EGCG na GCG.

Mu bushakashatsi bwakurikiyeho, byagaragaye ko Teadenol yari ifite ibikorwa by’ibinyabuzima nko guteza imbere ururenda rwa adiponectine, kubuza poroteyine tyrosine fosifata 1B (PTP1B) no kwera, byashimishije abashakashatsi benshi. Adiponectine ni polypeptide yihariye cyane ya tipusi ya adipose, ishobora kugabanya cyane indwara ziterwa na metabolike muri diyabete yo mu bwoko bwa II. Kugeza ubu PTP1B izwiho kuba intego yo kuvura diyabete n'umubyibuho ukabije, byerekana ko Teadenol ifite hypoglycemic n'ingaruka zo kugabanya ibiro.

Muri iyi nyandiko, hasuzumwe ibiyikubiyemo, biosynthesis, synthesis hamwe na bioactivite ya Teadenol mu cyayi cya ferment ya mikorobe yasubiwemo, hagamijwe gutanga ubumenyi bwa siyansi hamwe n’amahame agenga iterambere no gukoresha Teadenol.

TA Ishusho ifatika

01

Kumenya Teadenol mucyayi cya ferment ya fer

Nyuma yuko Teadenol ibonetse muri Aspergillus SP (PK-1, FARM AP-21280) icyayi gisembuye bwa mbere, tekinike ya HPLC na LC-MS / MS yakoreshejwe mu kwiga Teadenol mu cyayi gitandukanye. Ubushakashatsi bwerekanye ko Teadenol ibaho cyane mu cyayi cya ferime.

TA, Chromatogramu y'igituntu

Spect Mass spectrometrie yicyayi cya ferment ya fer na TA na TB

Aspergillus oryzae SP.PK-1, FARM AP-21280, Aspergillus oryzae sp.AO-1, ​​NBRS 4214, Aspergillus awamori sp. , NBRS 4122), Eurotium sp. Ka-1, FARM AP-21291, Ubushakashatsi butandukanye bwa Teadenol bwagaragaye mu cyayi gisembuye Kippukucha, Saryusoso, Yamabukinadeshiko, Suraribijin na Kuroyamecha, gentoku-cha yagurishijwe mu Buyapani, no mu cyayi gitetse cya Pu erh, icyayi cya Liubao na Fu Brick icyayi cy'Ubushinwa.

Ibiri muri Teadenol mu cyayi bitandukanye biratandukanye, bikaba bivugwa ko biterwa nuburyo butandukanye bwo gutunganya hamwe na fermentation.

Ubundi bushakashatsi bwerekanye ko ibikubiye muri Teadenol mu bibabi byicyayi bitarimo gutunganya mikorobe, nkicyayi kibisi, icyayi cyumukara, icyayi cya oolong nicyayi cyera, byari bike cyane, cyane cyane munsi yumupaka. Ibiryo bya Teadenol mumababi atandukanye yicyayi bigaragara mumbonerahamwe 1.

02

Bioactivite ya Teadenol

Ubushakashatsi bwerekanye ko Teadenol ishobora guteza imbere kugabanya ibiro, kurwanya diyabete, kurwanya okiside, kubuza ikwirakwizwa rya kanseri ya kanseri ndetse n’uruhu rwera.

Teadenol A irashobora guteza imbere ururenda rwa adiponectin. Adiponectine ni peptide ya endogenous isohoka na adipocytes kandi yihariye cyane ya tipusi. Ifitanye isano ribi cyane na visceral adipose tissue kandi ifite anti-inflammatory na anti-atherosclerotic. Teadenol A rero ifite ubushobozi bwo kugabanya ibiro.

Teadenol A irabuza kandi kwerekana protein tyrosine phosphatase 1B (PTP1B), Fosifata ya tirozine ya reseptor ya tyrosine ya fosifata mu muryango wa protein tyrosine phosphatase, igira uruhare runini mu kumenyekanisha insuline kandi kuri ubu ikaba izwi nk'intego yo kuvura diyabete. Teadenol A irashobora kugenzura neza insuline muguhagarika imvugo ya PTP1B. Hagati aho, TOMOTAKA n'abandi. yerekanye ko Teadenol A ari Ligand ya minisiteri ndende ya aside irike yakira GPR120, ishobora guhuza no gukora GPR120 kandi igateza imbere gusohora imisemburo ya insuline GLP-1 mu ngirabuzimafatizo zo mu nda STC-1. Glp-1 ibuza ubushake bwo kurya no kongera insuline, byerekana ingaruka zo kurwanya diyabete. Kubwibyo, Teadenol A ifite ingaruka zishobora kurwanya antiyabete.

Indangagaciro za IC50 z'igikorwa cyo gusya DPPH hamwe na superoxide anion radical scaringing ya Teadenol A yari 64.8 μg / mL na 3,335 mg / mL. Indangagaciro za IC50 z'ubushobozi bwa antioxydeant hamwe na hydrogène yo gutanga ni 17,6 U / mL na 12 U / mL. Byerekanwe kandi ko ikayi yicyayi irimo Teadenol B ifite ibikorwa byinshi byo kurwanya ikwirakwizwa rya kanseri ya HT-29, kandi ikabuza selile kanseri ya HT-29 kongera urugero rwa caspase-3/7, caspase-8 na Caspase -9, reseptor urupfu ninzira ya mitochondrial apoptose.

Byongeye kandi, Teadenol nicyiciro cya polifenole ishobora kwera uruhu muguhagarika ibikorwa bya melanocyte hamwe na synthesis ya melanin.

03

Synthesis ya Teadenol

Nkuko bigaragara mubyavuye mu bushakashatsi mu mbonerahamwe ya 1, Teadenol mu cyayi cya fermentation ya mikorobe ifite ibintu bike kandi nigiciro kinini cyo gukungahaza no kwezwa, bikaba bigoye guhaza ibikenewe mubushakashatsi bwimbitse no guteza imbere porogaramu. Kubwibyo, intiti zakoze ubushakashatsi kuri synthesis yibintu nkibi biva mubyerekezo bibiri bya biotransformation na synthesis.

WULANDARI n'abandi. yatewe na Aspergillus SP (PK-1, FARM AP-21280) mugisubizo kivanze cya EGCG na GCG. Nyuma yibyumweru 2 byumuco kuri 25 ℃, HPLC yakoreshejwe mu gusesengura imiterere yumuco. Habonetse Teadenol A na Teadenol B. Nyuma, Aspergillus oryzae A. Awamori (NRIB-2061) na Aspergillus oryzae A. Kawachii (IFO-4308) batewe mu ruvange rwa autoclave EGCG na GCG, bakoresheje uburyo bumwe. Teadenol A na Teadenol B byagaragaye muburyo bwombi. Ubu bushakashatsi bwerekanye ko guhindura mikorobe ya EGCG na GCG bishobora kubyara Teadenol A na Teadenol B. INDIRIMBO nibindi. yakoresheje EGCG nk'ibikoresho fatizo kandi yatewe na Aspergillus sp kugirango yige uburyo bwiza bwo gukora Teadenol A na Teadenol B n'umuco utuje kandi ukomeye. Ibisubizo byerekanye ko CZapEK-DOX yahinduwe irimo 5% EGCG na 1% yifu yicyayi kibisi byatanze umusaruro mwinshi. Byagaragaye ko kongeramo ifu yicyayi kibisi bitagize ingaruka ku buryo butaziguye ku musaruro wa Teadenol A na Teadenol B, ariko ahanini byatumye ubwiyongere bwa biosynthase burimo. Mubyongeyeho, YOSHIDA n'abandi. synthesize Teadenol A na Teadenol B kuva phloroglucinol. Intambwe zingenzi za synthesis yari asimmetric α -aminoxy catalitike reaction ya organic catalitiki aldehydes hamwe na allyl intramolecular allyl isimbuza palladium-catisale fenol.

Mic Microscopi ya electron ya fermentation yicyayi

04

Kwiga gusaba Teadenol

Bitewe nibikorwa byingenzi byibinyabuzima, Teadenol yakoreshejwe mubuvuzi, ibiryo nibiryo, kwisiga, reagents zo gutahura nizindi nzego.

Hariho ibicuruzwa bifitanye isano birimo Teadenol mu murima wibiribwa, nkicyayi cyabayapani Slimming Tea hamwe nicyayi cya polifenol. Byongeye, Yanagida n'abandi. yemeje ko ibivamo icyayi birimo Teadenol A na Teadenol B bishobora gukoreshwa mugutunganya ibiryo, ibiryo, inyongeramusaruro, ibiryo by'amatungo hamwe no kwisiga. ITO n'abandi. yateguye ibintu byingenzi byuruhu birimo Teadenol ningaruka zikomeye zo kwera, kubuza radical kubuntu ningaruka zo kurwanya inkari. Ifite kandi ingaruka zo kuvura acne, kuvomera, kongera imikorere ya barrière, kubuza uV ikomoka kumuriro hamwe nibisebe birwanya umuvuduko.

Mubushinwa, Teadenol yitwa icyayi cya fu. Abashakashatsi bakoze ubushakashatsi bwinshi ku bivamo icyayi cyangwa formulaire irimo icyayi cya fu A na Fu icyayi B mu rwego rwo kugabanya lipide yamaraso, kugabanya ibiro, isukari yamaraso, hypertension no koroshya imiyoboro yamaraso. Icyayi cyiza-cyicyayi Cyiza kandi cyateguwe na Zhao Ming nabandi. irashobora gukoreshwa mugutegura imiti ya antilipide. We Zhihong n'abandi. yakoze capsules yicyayi, ibinini cyangwa granules zirimo anhua icyayi cyijimye cya Fu A na Fu B, gynostema pentaphylla, Rhizoma orientalis, ophiopogon nibindi bicuruzwa bivura imiti nibiryo, bifite ingaruka zigaragara kandi zirambye mukugabanya ibiro no kugabanya lipide kubwoko bwose bw'umubyibuho ukabije. abantu. Tan Xiao 'ao yateguye icyayi cya fuzhuan hamwe na fuzhuan A na Fuzhuan B, byoroshye kwinjizwa numubiri wumuntu kandi bigira ingaruka zigaragara mukugabanya hyperlipidemiya, hyperglycemia, hypertension no koroshya imiyoboro yamaraso.

05

“Ururimi

Teadenol ni b-ring fission catechin ikomoka mucyayi cya ferment ya fer, ishobora kuboneka muguhindura mikorobe ya epigallocatechin gallate cyangwa muri synthesis yose ya floroglucinol. Ubushakashatsi bwerekanye ko Teadenol ikubiye mu cyayi gitandukanye cya mikorobe. Ibicuruzwa birimo icyayi cya Aspergillus Niger, icyayi cya Aspergillus oryzae, icyayi cyasembuwe, icyayi cya ferment ya Sachinella, icyayi cya Sachinella, Kippukucha (Ubuyapani), Saryusoso (Ubuyapani), Yamabukinadeshiko (Ubuyapani), Suraribijin (Ubuyapani), Kuroyamecha (Ubuyapani), Gentokame cha (Ubuyapani), Awa-Bancha (Ubuyapani), Goishi-cha (Ubuyapani), icyayi cya Pu 'er, icyayi cya Liubao n'icyayi cya Fu Brick, ariko ibiri muri Teadenol mu cyayi bitandukanye biratandukanye cyane. Ibiri muri Teadenol A na B byari hagati ya 0.01% na 6.98% na 0.01% kugeza 0.54%. Muri icyo gihe, icyayi cya oolong, cyera, icyatsi n'icyatsi cyirabura ntabwo kirimo ibyo bintu.

Kubijyanye nubushakashatsi bugezweho, ubushakashatsi kuri Teadenol buracyari buke, burimo gusa inkomoko, ibirimo, biosynthesis hamwe ninzira nyabagendwa yose, kandi uburyo bwibikorwa niterambere hamwe nogukoresha biracyakenewe ubushakashatsi bwinshi. Hamwe nubushakashatsi burenzeho, ibice bya Teadenols bizagira agaciro gakomeye ko kwiteza imbere hamwe nibisabwa mugari.