Icyayi ni kimwe mu binyobwa bitatu by'ingenzi, bikungahaye muri polyphenol, hamwe na antioxidant, anti-kanseri, anti -rus, hypoglycemic, ibikorwa bya hypoglycemic n'ibindi bikorwa hamwe n'ibindi bikorwa by'ubuzima. Icyayi gishobora kugabanywamo icyayi kidasemburwa, icyayi gisemburwa hamwe nicyayi cya nyuma ukurikije ikoranabuhanga ryo gutunganya nurwego rwa fermentation. Icyayi cya nyuma cyerekeza ku cyayi gifite uruhare rwa mikorori mu mutwe, nka PU 'er yatetse icyayi, icyayi cya FU, rwa Shubao, cya Shubaoso, ya Kuroyamera na Kuroyamera bakoze mu Buyapani. Iyi mikorobe isenyutse gukundwa nabantu bafite ingaruka zubuzima bwabo nko kugabanya ibinure byamaraso, isukari yamaraso na cholesterol.

Nyuma ya mikorobe, polyphenol yicyayi mu cyayi yahinduwe na enzymes na polyphenol nyinshi hamwe ninzego nshya. Teadenol A na Teadenol B ni kogosha Polyphenol byitaruwe icyayi feri hamwe na aspergills sp (pk-1, umurima AP-21280). Mu bushakashatsi bwakurikiyeho, byagaragaye mu cyayi kinini. Teadenol ifite stereomers ebyiri, Cis-Teadnol A na Trans-Teadenol B.4h12O6, Uburemere bwa Teadenol bwerekanwe ku gishushanyo 1. CamChins Corcess. Teadenol A na Teadenol B irashobora kuba biosynthesied kuva egcg na gcg.

Mu bushakashatsi bwakurikiyeho, wasangaga ko Teadenoli yakoze ibinyabuzima nko guteza imbere ubushishozi bwa Adiponectin, bubuza Tyrosine ya Prosphinectine 1b (PTP1B) imvugo no kwera, bikurura abashakashatsi benshi. Adiponectin numusagitiye cyane cyane kugirango ahishe tissue, ishobora kugabanya cyane indwara ya metabolike muri diyabete ya II. PTP1B kuri ubu yamenyekanye nkintego yo kuvura diyabete n'umubyibuho ukabije, byerekana ko teadels ifite hypoglycemic n'ingaruka zo kugabanya ibiro.

Muri iyi mpapuro, kumenya ibinyabuzima, ibinyabuzima byose hamwe na bonthesite bya bioirelite muri mikorobe yasubiwemo, kugirango batange ishingiro rya mikorobe yashizwemo, kugirango batange ishingiro rya mikorobe yashizwemo, kugirango batange ishingiro rya mikorobe yashizwemo, kugirango batange ishingiro rya mikorobe yasubiwemo, kugirango batange ishingiro rya mikorobe yashizwemo, kugirango batange ishingiro rya mikorobe yasuzumwe, kugirango tutange ishingiro rya mikorobe yasuzumwe, kugirango tutange ibyatsi bya mikorori ryasuzumwe, kugirango tutange ibyakozwe na mikorobe byafashwe, hagamijwe gutanga ishingiro rya siyansi na siyansi hamwe niterambere ryiterambere no gukoresha teadenoli.

▲ ta ishusho yumubiri

01

Gutahura icyayi muri mikorobe ya feribial

Nyuma ya Teadnol yabonetse muri aspergills sp (pk-1, umurima ap-21280) Icyayi cyambere, hplc na the tekinike yakoreshejwe mugukoresha icyayi muburyo butandukanye bwicyayi. Ubushakashatsi bwerekanye ko teadnol ibaho cyane cyane muri microbial fermential.

▲ Ta, Tb Amashanyarazi ya chromatogram

▲ Spectrometry ya Microbial Icyayi na Ta na Tb

Aspergillus oryzae sp.pk-1, Umurima AP-21280, Aspergillus Oryzae SP.SK-NBRGINUS ORYJORI SP.SK-ASPERGILUS BO SP. Ka-1, Umurima AP-21291, Ibitekerezo bitandukanye bya Teadenoli byagaragaye mu cyayi cya feri kippukucha, Sarusoso, Yamabukari na Kuroyamecha, maze icyayi cyatetse cya PU Erh, Icyayi cya Buro, cya FU

Ibiri muri Teadenoli mubintu bitandukanye biratandukanye, bikaba byavuzwe kugirango biterwe nibibazo bitandukanye byo gutunganya no gutwika.

Ubundi buryo bwerekanaga ko ibikubiye muri Teadenoli mumababi yicyayi adafite imiterere ya microbial, nkicyayi cyirabura, icyayi cya oolong nicyayi cyera, ahanini byari bike cyane. Teadnol ibirimo mumababi yicyayi atandukanye yerekanwa mu mbonerahamwe ya 1.

02

Bioatite ya teadnol

Ubushakashatsi bwerekanye ko teadnol ishobora guteza amava, kurwanya diyabete, kurwanya ikananga, ibuza ikwirakwizwa ry'ingirabuzimafatizo no ku ruhu rwera.

Teadengol A irashobora guteza imbere imbaraga za adiponectin. Adiponectin ni endogenous peptide ihinduka na adipocytes kandi yihariye cyane kugirango uhishe tissue. Birafitanye isano cyane no kwerekana tisseral tissue kandi ifite anti-induru na anti-athesclerotic. Teadnol rero afite ubushobozi bwo kugabanya ibiro.

Teadenol A ibuza imvugo ya tyrostine tyrosine ya prophine ya prosphahase 1b (PTP1B), igira uruhare runini kuri tyroskine tyleyine tyleyine ya possikari muri poskrosine kandi ikaba izwi nkintego yo kuvura diyabete. Teadenol A irashobora kugenzura insuline muguhagarika imvugo ya PTP1B. Hagati aho, tomotaka et al. yerekanye ko Teadenol A ni ligand ya acide maremare acide reseptor gPR120, ishobora guhambira muri GPR-80 no guteza imbere ibanga rya insuline glp-1 muri endocrine stc-1 Ingirabuzimafatizo. GLP-1 ibuza ubushake kandi wongera imbaraga za insuline, kwerekana ingaruka zo kurwanya diyabete. Kubwibyo, Teadenol A ifite imbaraga za antidiabete.

Indangagaciro za IC50 za DPPHS hamwe na superoxide anions anions radenging ibikorwa bya teadnol A yari 64.8 μg / ml na ml, ml. Indangagaciro za IC50 za Antioxident Antioxydant nubushobozi bwo gutanga hydrogen bwari 17.6 U / ML na 12 U / ML. Ryerekanwe kandi ko gukuramo icyayi cya teadnol b gifite ibikorwa byimbitse byo kurwanya HT-29 Ingirabuzimafatizo CYA KAZA-3/7, 8 na Caspuase - 9 na Caspaseri Urupfu rwa Apoptose.

Byongeye kandi, teadnol ni icyiciro cya polyphenol gishobora kuzungura uruhu ubuza ibikorwa bya melanocyte na melanin synthesis.

03

Synthesis ya teadnol

Nkuko bigaragara mumakuru yubushakashatsi mu mbonerahamwe ya 1, Teadenoli muri mikorobe ya mokoro ifite ibintu bike hamwe nigiciro kinini cyo gukungahaza no kwezwa, biragoye guhaza ibikenewe mubushakashatsi bwimbitse no guteza imbere ubushakashatsi bwimbitse no guteza imbere ubushakashatsi bwimbitse no guteza imbere ubushakashatsi bwimbitse no guteza imbere ubushakashatsi bwimbitse no guteza imbere ubushakashatsi bwimbitse no guteza imbere ubushakashatsi bwimbitse no guteza imbere ubushakashatsi bwimbitse no guteza imbere ubushakashatsi. Kubwibyo, intiti zakoze ubushakashatsi kuri synthesis yibintu biva mubyerekezo bibiri bya biotranformation na synthesique sympesis.

Wulandari et al. ASPOCILS ASPERGILUS SP (PK-1, Ubuhinzi AP-21280) Mubisubizo bivanze byamagambo ya egcg na gcg. Nyuma yibyumweru 2 umuco kuri 25 ℃, HPLC yakoreshejwe mugusesengura imiterere yumuco. Teadenol A na Teadenol B bagaragaye. Nyuma, aspergillus oryzae A. Awamori (nari-0661) na aspergill oryzae (ito-4308) yatunganijwe mu ruvange rwa autoclave egcg na gcg, bakoresheje uburyo bumwe. Teadenol A na Teadnol B bagaragaye muburyo butari bwo. Ubu bushakashatsi bwerekanye ko guhinduka kwa mikorobe ya egiccg na gcg bishobora kubyara icyayi A na Teadenol B. Indirimbo et al. yakoresheje egcg nkibikoresho fatizo na aspergills sp kugirango wige ibihe byiza kuri Teadenol a na Teadenol B numuco wamazi numuco wamazi numuco ukomeye. Ibisubizo byerekanaga ko igipimo cya Czapek-dox kirimo egcg 5% na 1% ifu yicyayi yagira umusaruro mwinshi. Yasanze hiyongereyeho ifu y'icyayi itigeze igira ingaruka ku musaruro wa Teadenol A na Teadenol B, ariko cyane cyane zashishikarije ubwiyongere bw'umubare wa biosynthase. Byongeye, Yoshida et al. Synthesid Teadenol A na Teadenol B kuva Phloroglucinol. Intambwe z'ingenzi za Synthesis zari asimetero α - kaminoxy catalytique ya kataleti kama kama hamwe no gusimbuza insanganyamatsiko ya palladium ya palladium-cataliz.

▲ Microscople ya Electrod yicyayi

04

Kwiga Gusaba Teadnol

Bitewe nibikorwa byayo byingenzi, teadnol byakoreshejwe muri faruceti, ibiryo n'ibiryo, kwisiga, guhitamo, guhitamo reagent nizindi nzego.

Hano hari ibicuruzwa bifitanye isano birimo teadnol mukibuga cyibiribwa, nkicyayi cya kibaya cyayapani na polyphenols ya felphenoles. Byongeye kandi, yanagida et al. yemeje ko inkuta z'icyayi zirimo Teadenol A na Teadel B zishobora gukoreshwa mu gutunganya ibiryo, gutondekanya, amafaranga y'ubuzima, ibiryo by'inyamaswa no kwisiga. Ito et al. yateguye umukozi wibanze ku ruhu arimo teadnol hamwe ningaruka zikomeye zera, kubuza gukabije kubuza no kurwanya inketi. Ifite kandi ingaruka zo kuvura acne, gucogora, kuzamura imikorere ya bariyeri, kubuza gutwika uv-bivanze no kurwanya igituba.

Mu Bushinwa, Teadenol yitwa PU Icyayi. Abashakashatsi bakoze ubushakashatsi bwinshi ku cyayi cyangwa ibikomokaho birimo icyayi cya fu a no mu cyayi cy mu buryo bwo kugabanya lipids yo kugabanya amaraso, kugabanya ibiro, isukari mu maraso, hypersension no koroshya imiyoboro y'amaraso. Icyayi-cyera icyayi cya feri kandi cyateguwe na Zhao MING et al. irashobora gukoreshwa mugutegura imiti ya antilipid. HI Zihong et al. yakoze icyayi, ibinini cyangwa granules birimo icyayi cyijimye cya fu a na fu b, ibikomoka ku bicuruzwa bifatika, bikaba bifite ingaruka mbi kandi bikaranze kandi bigabanya lipdid kubantu bose bafite umubyibuho ukabije. Tan Xiao 'ao yateguye icyayi cya Fuzhuan hamwe na Fuzhuan A na Fuzhuan B, byoroshye kwishora mu kugabanya hyperlipidemia, hyperglycemia, hyperglycemia, hypertemia, hyperdension no yoroshye imiyoboro y'amaraso.

05

"Ururimi

Teadenol ni B-impeta Fention Corventin Ibikomokaho muri microbial bihari, ishobora kuboneka kuva ihinduka rya mikorobe ya epigallocatechin cyangwa muri synthesis yose ya phloroglucinol. Ubushakashatsi bwerekanye ko teadnol ikubiye mu murima zitandukanye. Ibicuruzwa birimo Aspergillus Niger Ferment Cyera, Aspergillus Oryzae Icyayi Cyane, Saristoso (Ubuyapani), Umuyapani), Umupapani), Mantok U-Char-Bancha (Ubuyapani), Goishi-cha (Ubuyapani), PU 'er icyayi, icyayi cya Buubao na FU amatafara ya Fu Ibiri muri Teadenol A na B byatandukanije na 0.01% kuri 6.98% na 0.01% kuri 0.54%. Mugihe kimwe, oolong, cyera, icyatsi nicyatsi kibisi ntabwo birimo ibi.

Ku bijyanye n'ubushakashatsi kuri ubu, ubushakashatsi kuri teadnol buracyagarukira, burimo inkomoko gusa, ibirimo, inzira nyabagendwa n'inzira nyabagendwa, hamwe n'uburyo bwaho buracyakeneye ubushakashatsi bwinshi. Hamwe nubushakashatsi, ibigo bya teadnol bizagira agaciro gakomeye hamwe nibisabwa.