Umi edemede
9,10-anthraquinone (AQ) bụ ihe ọghọm nke nwere ike ịnwe ụgbọ ala ma na-eme na ti n'ụwa niile. Oke mkpofu kachasị (Mrl) nke AQ na tii Europe na-egosi (EU) bụ 0.02 mg / kg. Isi mmalite nke AQ na nhazi tii na nnukwu ọkwa nke ihe omume ya bụ usoro mgbanwe AQ na Calcographyography (Tandem uka Oversetrometry (GC-MS / MS). E jiri ya tụnyere eletrik dị ka okpomọkụ okpomọkụ na-acha akwụkwọ ndụ, AQ mụbaa site na nha (nke na-eme ihe eji eme ya, dị elu karịa 0.02 mg / kg, ebe ọkwa AQ na gburugburu obibi. A hụrụ otu usoro a na Oolong tii n'okpuru okpomọkụ. Nzọụkwụ ndị dị na kọntaktị epupụta na akpị, dị ka ndozi na ihicha, a na-ahụta dị ka usoro mmepụta nke AQ na-arụ ọrụ. Ọkwa nke AQ mụbara na oge kọntaktị na-ebili, na-atụ aro na enwere ike inweta na aq poyel na tii a nwere ike ibute ya site na ajị anụ ahụ na-ebute na a. Nlekọta anọ dị iche iche na-arụ ọrụ ma ọ bụ Call dị iche iche, sitere na isi iyi ọkụ na 50.0% na 5.0% -35.0% maka nchọpụta na karịa ọnụego AQ. Na mgbakwunye, a na-ahụ ọdịnaya AQ kachasị nke 0.064 mg / kg na ngwaahịa tii na tii dị ka isi iyi ọkụ, na-egosi na ag nwere ike itinye ya na kol.
Keywords: 9,10-anthraqinone, nhazi tii, unyi, mmerụ isi
Ndubata
Tii emepụtara site na akwụkwọ nke osisi camellen na-eme mmehie (L.) O. Kuntze, bụ otu n'ime ihe ọverasaụ na-ewu ewu na uru ahụike ya na-enye ume ọhụrụ. Na 2020 ụwa niile, tii mepụtara amụbaala na nde mitric 5,972, nke bụ okpukpu abụọ n'ime afọ iri abụọ gara aga [1]. Dabere na ụzọ nhazi dị iche iche, gụnyere tii na-acha akwụkwọ ndụ akwụkwọ ndụ, tii brai, tii, oolong tii, oolong tii, na-acha ọcha tii na tii (2,3]. Iji hụ na ogo na nchekwa nke ngwaahịa, ọ dị ezigbo mkpa iji nyochaa ọkwa nke mmetọ ma kọwaa mmalite.

Ifyingmata isi mmalite nke mmerụ ahụ, dị ka pesti nzuzo, ọla dị arọ na mmetọ ndị ọzọ dị ka polyclic arocarbons (pahs), bụ ihe dị mkpa iji chịkwaa mmetọ. Padgba ya ịgbapụta mmiri ọgwụ na tii, yana ikuku na-efe efe kpatara site na tii ubi, bụ isi mmalite nke ahịhịa na tii [4]. Nnukwu ọla nwere ike imubanye na tii ma na-eduga nsị, nke a na-enweta site na ala, fatịlaịza na ikuku (5-7]. Banyere mmetọ ndị ọzọ na-apụta na tii na-atụghị anya ya, ọ bụ ihe siri ike ịchọpụta n'ihi usoro dị mgbagwoju anya nke setịpụrụ tii na-egosi na certing, nhazi, mbukota na ụgbọ njem. Pahs na tii sitere na nkwenye nke ike ụgbọala na ngwakọta nke a na-eji n'oge nhazi nke tii, dị ka nkụ na unyi na vetiiti [8-10].

N'oge kol na nkụ na-efe efe, mmetọ dị ka e guzobere igwe carbon ka [11]. N'ihi ya, ọ ga-abụ na-enwe ike ịgbachitere mmetọ ndị a a kpọtụrụ aha na-eme na ngwaahịa a haziri aha, dị ka ọka, smoked ngwaahịa, na oke okpomọkụ, na-etinye ihe iyi egwu maka ahụike mmadụ [12,13]. A na-enweta pahs na-esite site na nsị nke romatilization nke flus onwe ya, ebe a na-emegharị ọnọdụ dị elu na mmeghachi omume dị n'etiti radicals [14]. Oké ifufe, oge, ọdịnaya oxygen bụ ihe dị mkpa na-emetụta ntụgharị nke pahs. Site na mmụba nke okpomọkụ, nke Phs ọdịnaya buru ụzọ mụọ ma belata ya, na ọnụego elu ahụ mere na 800 Celsius C; Ihe ndị ahụ na-eme ka ọ bụrụ nke ọma na-abawanye na ịba ụba na ikuku oxygy, ma ọ bụ mmụba nke oxygen na usoro ndị ọzọ [15-17].

9,10-anthraquinone (AQ, nke: 84-65-1, 1), oxygen nwere oxygen nke pahs, mejupụtara usoro usoro atọ ahụ. Edepụtara ya dị ka Carcinogen (otu pụrụ iche) site na ụlọ ọrụ mba ụwa maka nyocha na ọrịa kansa n'afọ 2014 [19]. AQ nwere ike nsi na Topomerase II gbabiri ma gbochie hydrolysis nke etiti (ATP) site na DNA Topormer na-enwe ike ibute ya, mmụba ma nwekwuo ọrịa kansa [20]. Ka mmetụta na-adịghị mma na ahụike mmadụ, AQ kachasị mma residue (MRL) nke 0.02 mg / kg na tii Europe. Dị ka ọmụmụ ihe anyị gara aga, a tụrụ aro itinye ego nke AQ dị ka isi mmalite dị ka ihe isi iyi mgbe Osisi Tei [21]. Ọzọkwa, dabere na nnwale nke Indonese Green na Black Fise, ọ gbanwere na an2 a tụrụ aro ka ọ bụrụ ihe a na-ahazi akụrụngwa dị ka otu ihe kpatara ya. Agbanyeghị, mmalite nke AQ na nhazi tii ka dị mkpa, ọ bụ ezie na ụfọdụ hypotheses nke ụzọ AQ kemịkal na-ekpebi ihe ndị dị mkpa na-emetụta ọkwa AQ.

Ọgụgụ 1. Memmo kemịkal nke aq.

Nyere nyocha nke AQ n'oge a na - eme ihe na - eme ihe na - eme ihe na - eme ka ọ dị na Nhazi AQ na - akọwapụta ya na usoro nhazi dị iche iche, nke na - enye aka iji kọwaa nsonaazụ zuru oke, usoro ihe ndapụta na ogo nke aQ na-akwụ ụgwọ na usoro nhazi tii.

Nsonaazụ
Usoro eji eme ihe
Ejinyere ya na omumu ihe omumu anyi gara aga, a na-etinye usoro mbata mmiri mmiri mmiri tupu ịmegharị GC-MS / ms iji meziwanye echiche ma nọgide na-ekwupụta echiche. Na fig 2B, usoro dị mma gosipụtara ọganihu dị mkpa na ọcha nke ihe nlele ahụ, ihe mgbaze ghọrọ ọkụ na agba. Na fig 2a, ụdị scanomi zuru ezu (50-350 m / z) dị iche iche, akara nke na-egosi na chromatograph obosara wepụrụ ya mgbe mmiri mmiri mmiri.

Ọgụgụ 2. (b) Mmetụta ọcha nke usoro ka mma.
Nkwado usoro, gụnyere linearnity, gbakee, oke nke ọtụtụ (na-eju anya site na tii 0.998, na ikuku nke 0,5 ruo 8 μg / m3.

A na-enyocha AQ na-enyocha ihe atọ dị na atọ dị n'etiti tii (0.05, 0.5, 1.5, 1.5, 3 μg / m3). Mgbake nke AQ na tii sitere na 77.78% ruo 113.02% na tii na-akọrọ, na RSD% dị ala karịa 15%. Mgbake nke AQ na sampless sitere na 78.47% ruo 117.06% na Rsd% n'okpuru 20%. A na-achọta itinye uche dị ka loq, nke dị 0,005 mg / kg na 0,5 μ / tii na igwe ikuku, n'otu n'otu. Dị ka edepụtara na tebụl 1, matrix nke tii ntakịrị mụbara nzaghachi AQ, na-eduga m 109.0% na 110.9%. Banyere matriks nke ihe nlele ikuku, m bụ 196.1%.

Ọkwa nke AQ n'oge nhazi akwụkwọ
Ebumnuche nke ịchọta nsonaazụ nke isi iyi ọkụ dị iche iche na gburugburu ebe a na-ahazi, a na-ekewa epupụta epupụta n'ime otu abụọ ma hazie iche iche na otu ụlọ ọrụ. Otu eletrik ewepụtara na ya na ọkụ eletrik, na nke ọzọ nwere kol.

Dị ka egosiri na fig 3, ọkwa AQ nwere ọkụ eletrik dị ka okpomọkụ nke okpomọkụ si 0.008 ruo 0.013 mg / kg. N'oge usoro ndozi, a na-eme mpempe akwụkwọ epupụta mere site na nhazi n'ime ite na elu okpomọkụ rụpụtara na AQ. Mgbe ahụ, ọkwa nke AQ a ka dị n'oge usoro na-agagharị n'agbanyeghị ọnwụ nke ihe ọ juiceụ juiceụ, na-atụ aro na usoro anụ ahụ nwere ike ọ gaghị emetụta ọkwa nke AQ na nhazi tii. Mgbe mbụ a na-asacha mmiri, ọkwa AQ mụbaa na 0.010 ruo 0.012 mg / n'arọ, wee nọgide na-ebili na 0.013 mg / n'arọ ruo na njedebe nke ịcha nkụ. PFS, nke pụtara ìhè gosipụtara ọdịiche na usoro nke ọ bụla, 1.03, 1.03, 1.08, 1.08 na ndozi, na-agagharị na ihicha, n'otu n'otu. Nsonaazụ PFS tụrụ aro na nhazi n'okpuru ike eletriki nwere obere mmetụta na ọkwa nke AQ na tii.

Ọgụgụ 3
N'ihe dị ka isi iyi ọkụ, ọdịnaya AQ na-abawanye ụba n'oge nhazi tii, na-enwu site na 0.008 ruo 0.038 mg / kg. 338.9% AQ bidoro n'usoro ndozi, erute 0.037 mg / kg, nke gafere nke Mrl nke European Union. N'oge na-agagharị na ogbo, ọkwa nke AQ ka mụbaa site na 5.8% n'agbanyeghị na ịnọ ebe dị anya site na igwe ndozi. Na ihicha na ihicha, ọdịnaya AQ mụbara obere ma ọ bụ belata ntakịrị. PFS na-eji kol dị ka isi ikuku na ndozi, na-akpochasị mbụ na ihicha bụ 439, 1.93, 0.93, na 1.05, n'otu n'otu,.

Iji nwetakwuo mmekọrịta dị n'etiti mmetọ aq na mmetọ aq, a na-akwụsịtụ ihe na-egosi (PMS) na fig. 4.

Ọgụgụ 4. Ọdị ọkwa nke AQ na gburugburu ọkụ eletrik na unyi. * Na-egosi ọdịiche dị mkpa na ọkwa AQ na-egosi na nlele (p <0.05).

Ọkwa nke AQ n'oge olong tii na-eme ka oolong tii, nke tumadi na Fujian na Taiwan, bụ ụdị tii e ji eme nri. Iji gaa n'ihu ịchọpụta usoro AQ na nsonaazụ nke ọkụ dị iche iche, otu akụkụ nke akwụkwọ ọhụrụ na ngwakọ ga-enwu ọkụ. Nhazi AQ na Oolong tii na-eji ngwa agha dị iche iche e gosipụtara na Fig-5. Maka nhazi ọkụ na-acha ọcha dị n'okpuru 0.005 mg / kg, nke yiri nke a ọkụ eletrik.

Ọgụgụ 5

Na kol dị ka isi iyi ọkụ, ọkwa AQ na usoro abụọ mbụ, na-akpọnwụ na ime akwụkwọ ndụ akwụkwọ ndụ, bụ otu ihe dị ka ya na ngwakọta ikuku na-apụ apụ. Agbanyeghị, usoro ndị a na-esote ruo mgbe edozichara gosipụtara nwayọ, nke na-egosi ọkwa AQ na 0.004 ruo 0.023 mg / kg. Ọkwa dị na ndọkpụ a na-agbaze ka ọ belata ruo 0.018 mg / n'arọ, nke nwere ike ịbụ n'ihi mfu ihe ọ juiceụ tii tii na-ewepụ ụfọdụ ihe mmetọ a. Mgbe nzacha ọkwa, ọkwa dị na ogbo a na-asacha na-amụba ruo 0.027 mg / n'arọ. Na-eme, na-eme akwụkwọ ndụ akwụkwọ ndụ, ndozi, na ihicha, pfs bụ 2.81, 1.66, 0.78, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, na 1.50, dika.

Ihe omume nke AQ na Tea Ngwa Ngwa nwere isi iyi dị iche iche

Iji chọpụta nsonaazụ nke Tii na-enwe ọdịnaya tii dị iche iche, na-egosi na ọkụ eletrik, yana ọ dị mfe iji mee ka ọ dị mfe iji ya mee ihe na-emepụta ihe A hụrụ 35.0% na njirisi nke kol. Imirikiti ndị na-enweghị atụ, ọkụ eletrik nwere ọnụ ọgụgụ dị ala na nke 56.4% na 7.7% n'otu n'otu, yana ọdịnaya kachasị nke 0.020 mg / kg.

Mkparita uka

Dabere na PFS n'oge nhazi abụọ nke ụlọ elu ọkụ, ọ doro anya na idozi ya bụ isi ihe na-eme ka mmụba nke tii tii nwere obere mmetụta na ọdịnaya nke AQ na tii. N'oge nhazi nke akwụkwọ ndụ akwụkwọ ndụ, colkpa conscurion mepụtara ọtụtụ ihe na usoro ndozi nke aq na-egosi na a na-eme ihe na-eme ka ọ bụrụ ihe nlereanya na-ese anwụrụ ọkụ [25]. Ọ dịtụ mmụba na ọdịnaya AQ n'oge ihe na-agagharị na-egosi na a na-eme ka ọ ghara imetụta aq ọ bụghị naanị na ọkwa nhazi, kamakwa na nhazi ebe a na-edebe ikuku. E jikwa mkpuchi dị ka okpomọkụ na-ete mmiri na ihicha ya, mana na usoro abụọ a na-abawanye ntakịrị ma ọ bụ belata ntakịrị. Enwere ike ịkọwa nke a site n'eziokwu ahụ bụ na ebe a na-asa mmiri na-ekpo ọkụ wepụtara tetumus nke ejikọtara ọnụ nke ọkụkọ. Iji chọpụta ebe nrụrụtọ ahụ, a na-enyocha ọkwa AQ na ikuku, na-ebute nnukwu ọdịiche dị n'etiti ụlọ ọrụ abụọ ahụ. Isi ihe kpatara nke a bụ na anụ ahụ eji eme ihe na ndozi, nke mbụ ihicha ma kpochapu steepụ ga-ewepụta AQ n'oge a na-ezughị ezu. A na-agbasa Aq ndị a na obere ụmụ aka nke soluds ma gbasasịa ikuku, na-ebuli ọkwa nke aQ na-ebuli ọkwa nke usoro ọmụmụ [15]. Ka oge na-aga, n'ihi nnukwu mpaghara tii a kapịrị ọnụ nke tii, ndị a na-akacha mmado wee biri n'elu akwụkwọ tii ahụ, na-ebute mmụba nke AQ na mmepụta. Ya mere, echere na ọgbụgba na-eduga na mbuso agha AQ na-eduga na nhazi tii, na-agba ụta ịbụ isi iyi nke mmetọ.

Banyere nhazi nke Oolong, AQ mụbara n'okpuru ebe obibi ma okpomọkụ, mana ọdịiche dị n'etiti isi mmalite abụọ ahụ ọkụ dị mkpa. Nsonaazụ ahụ tụkwara aro na ọkụ ọkụ na-arụ ọrụ dị ukwuu na ịba ụba AQ ọkwa maka mmụba AQ na nhazi Hoi na-arụ na PFS. N'oge oolong tii nwere ngwakọ ikuku nke mmanụ dị ka ihe na-acha ọkụ ọkụ, na-eme ka eletrik na-acha akwụkwọ ndụ, na-egosi na ikuku na-acha akwụkwọ ndụ, nwere ike belata ihe ize ndụ nke ịmepụta ihe mgbochi site na nhazi.

Banyere ule nlele, nsonaazụ a gosipụtara na ọnọdụ nke aq na-eme ka ọkụ eletrik karịa ọkụ eletrik na-agagharị na akwụkwọ tii na igwu waya ebe ọrụ. Agbanyeghị, ọ pụtara ìhè na eletrik bụ isi mmiri dị ọcha n'oge nhazi tii, a ka nwere igwe na-eme ka ọkụ si tii na-eji ọkụ ọkụ dị ka okpomọkụ. Ọnọdụ ahụ yiri ka e bipụtara ọrụ a na-emebu na mbụ nke mmeghachi omume nke mmeghachi omume nke mmadụ abụọ na Benzoquinnones dị ka ebumnuche kemịkalụ [23], a ga-enyocha ihe kpatara nke a.

Okwubibi

Na ọrụ a, isi mmalite nke aQ na-acha akwụkwọ ndụ akwụkwọ ndụ na olong tii sitere na usoro nke GC-MS / MS. Ihe nchoputa anyị kwadoro na isi iyi mmetọ dị elu nke ọkwa dị elu nke aq dị ọkụ na-eme site na nkụ, nke na-emetụtaghị usoro nhazi ya ma na-emetụta gburugburu ebe a na-emetụta gburugburu. N'adịghị ka a na-apụ apụ na ijikọkwa ọkwa, ebe mgbanwe dị na ọkwa nke AQ bụ ihe na-enweghị isi, bụ usoro a na-enwe, bụ usoro a na-enwe, bụ usoro a na-etinye na ego kọntaktsị n'etiti tii na ajị a. Ya mere, a na-atụ aro ka ọ dị ọcha na ọkụ eletrik dị ka isi iyi ọkụ na nhazi tii. Ọzọkwa, nsonaazụ nnwale gosipụtara na enweghị fumes na-emepụta site na ịhazi aq n'oge ọmụmụ ihe dị ọcha, a ga-enyocharịrị obere ihe ọhụụ.

AKỤRỤNGWA NA ỤZỌ

Regarcents, Chemicals na Ihe

Azụtara ọkọlọtọ (99.0%) zụrụ site na Dr. EHrenterfer Gmbh Company (Augustburg, Germany). Azụtara ọkọlọtọ dị n'ime (98.6%) site na C / D / N Ekotopes (Quebec, Canada). Sodium sodium sulfate (Na2SO4) na magnesium sulfate (MGSO4) (Shanghai, China). A na-eweta Florisil site na Welzhou Groparlọ ọrụ kemịkalụ (WAnsHOU, China). Azụtara mpempe akwụkwọ Mircrorro-iko (90 mm) site na ụlọ ọrụ Ahlstromö (Helsinki, Finland).

Nkwadebe nlele

A na-ahazi sai ahụ na ndozi, na-agagharị, na-agbacha akpa ma na-akpọgharị ya, na-eme ihe nlereanya tii (na-eme ka ọ dị ọhụrụ), ndozi, na ihicha. A na-anakọta ihe nlere site na usoro nke ọ bụla ugboro atọ na 100G mgbe agwakọta nke ọma. A na-echekwa ihe atụ niile na -20 Celsius C maka nyocha ọzọ.

A na-anakọta ihe nlere anya site na iko iko (90 mm) site na iji usoro ụda olu (PTTDAO, China) [27], na-agba ọsọ na 100 l / min maka 4 l.

A na-eme ihe nlere siri ike na 0.005 mg / kg, 0.500 mg / kg, 0.0 μ / «0.036 mg / kg (n'arọ (1.5) μg / m3 maka ikuku ikuku), 0.072 mg / kg (3.0 μg / m3 maka nlele ikuku) maka mpempe ihe iko, n'otu n'otu. Mgbe ị na-ama jijiji, ndị nlele niile fọdụrụ maka 12 h, mịpụ ngbanwe na nhicha dị ọcha.

Enwetara mmiri ọdịnaya mmiri site na ihe nlereanya mgbe ị gwakọtara nzọụkwụ, kpo oku na 105 Celsius ma na-emegharị ya site na ibu ya site na ibu ya site na iji kpo oku.

Mwepu na nhicha

Emere tii: mmịpụta na nsị nke AQ site na usoro onyonyo sitere na usoro e bipụtara si Wang Et al. Ọtụtụ mmegharị ọtụtụ ihe [21]. Na nkenke, 1.5 g nke sai tii tii na 30 μl d8-aq (kg) ma tinye ya na mmiri iri ato, mgbe ahu juputara na mmiri 1.5 my na-eguzo 30 min. 15 ml 20% acetone na n-herxane na telepleụ tii na sooro maka 15 min. Mgbe ahụ nlele ndị ahụ na-emegharị ya na 1.0 g mgso4 maka 30 s, na centrifed maka 5 min, na 11,000 rpm. Mgbe a kwagara ya na flass 100 ml ube dị elu, 10 ml nke akuku akuku akuku ihe fọrọ nke nta ka ọ bụrụ nkụ n'okpuru oghere na 37 Celsius C. 5 ml 2.5% acetone na n-herxane re-ichusa nsị na flass nke nwere oghere maka ime ka ọ dị ọcha. Ogwe iko (10 cm × 0.8 cm) nwere ala iko na 2G florisil, nke dị n'etiti abụọ na 2 cm na2SO4. Mgbe ahụ 5 ml nke 2.5% acetone na n-hexan na kọlụm ahụ. Mgbe e detuchara usoro mgbanwe, a kpọlitere AQ ugboro atọ na 5 ml, 10 ml nke 2.5% acetone na n-hexane. A na-ebugharị ihe ndị mejupụtara ya na flasks na-acha uhie uhie ma wepụ ihe fọrọ nke nta ka ọ bụrụ nkụ n'okpuru oghere na 37 Celsius C. A na-edozigharị ahịhịa ndị a mịrị amị na 1 ml nke acetone na hexan na-esochi ya site na nzacha site na nzacha 0.22 μm pora nha. Mgbe ahụ, a gwakọtara ngbanwe ejikọtara ya na acetonitrile n'olu olu nke 1: 1. Followinggbaso nzọụkwụ na-akpali akpali, a na-eji supernant ndị ahụ maka nyocha GC-MS / MSS.

Mpempe akwụkwọ ikuku: ọkara nke mpempe akwụkwọ ahụ, nke a na-awụpụ 18 μl d8-aq (n'arọ), na-mikpuru na 15 ml acetone na n-hexane, mgbe ahụ, mgbe ahụ soonited na 15 min. A na-ewepụ akụkụ ahụ akụkụ ahụ na centrifugation na 11,000 rpm maka min na a na-eji ahịhịa dị elu. A na-ebugharị usoro organic niile dị ka a kpọnwụọ n'okpuru oghere na 37 Celsius C. 5 ml nke 2.5% acetone na hexan resacsodeved e websacts maka ịdị ọcha n'otu ụzọ ahụ dị ka e si ele saiti.

Nnyocha GC-MS / MSS

A na-eji Mkpokọta 450 na-eme ihe dị iche iche 300 Tandam 300 nke Walnut Creek, Cornut Creek, CA, USA) na-eme nyocha AQ na ụdị ọrụ MSSTSTSTSSSTS 6.9.3. A na-eji mgbanwe dị iche iche na kọlụm dị na kọlụm (5 m × 0.25 mm × 0.25 × 0.25 μm) maka nkewa chromatographic. Gas gas, Helium (> 99.999%) Ọnọdụ okpomọkụ malitere site na 80 Celsius C ma jide 1 min; mụbara na 15 Celsius C / Min na 240 Celsius C, wee ruru 260 Celsius C na 20 Celsius C / min na ejidere 5min. Ọnọdụ okpomọkụ nke Ion Isi Iyi bụ 210 Celsius C, yana ahịrị windo nke 280 Celsius. Olu ogwu ahu bu 1.0 μl. A na-egosi ọnọdụ MRM na Isiokwu 3.

A na-ejikwa mmanụ ụgbọ ala 8890 na-eme ihe nkiri 7000D nke Quafutuole (Alilent, Stevens Creek, ca, USA) na-enyocha mmetụta dị ọcha na Mashunter Version Mmetụta na Mashunter Version 10.1. Ejiri kọlụm J & W HC-5m-5m (3 m × 0.25 mm × 0.25 ×) Ejiri ya maka nkewa chromatographic. Gas gas, helium (> 99.999%), na-aga n'ihu nke 2.25 ml / min nwere gas gas nke nitrogen (> 99.999%). E meziri ọnọdụ okpomọkụ nke eion isi na 280 Celsius C, otu dị ka ogo mbufe. Ọnọdụ okpomọkụ malitere site na 80 Celsius C ma enwere ya maka 5 min; MGBE 15 Celsius C / Min ga-eme ka 240 Celsius C, wee ruru 280 Celsius C / Min ma jigide maka 5 min. A na-egosi ọnọdụ MRM na Isiokwu 3.

Nnyocha Statistical
A na-edozi ọdịnaya AQ na akwụkwọ ọhụrụ ka a kpọọ ihe dị ka ihe ọkụkụ site na nke na-ekenye ya ma nyochaa ọkwa AQ n'oge nhazi.

A na-enyocha mgbanwe nke AQ na sọọsọ Microsoft Excel na IBM SPSS 20.

Ejiri nhazi ihe iji kọwaa mgbanwe dị na AQ n'oge nhazi tii. PF = RL / RF, ebe RF bụ ọkwa AQ tupu nhazi nzọụkwụ na Rl bụ ọkwa AQ mgbe nhazichara. PF na-egosi mbelata (PF <1) ma ọ bụ mmụba (PF> 1) na AQ

M na-egosi mbelata (m <1)

M = (Slopematrix / Slopesolvent - 1) × 100%

Ebe slopematrix bụ mkpọda nke mgbaze CACIX - dabara adaba na matriks, slopent bụ mkpọda nke usoro mmezi.

Inabata
Sayensị na teknụzụ dị mkpa na teknụzụ dị na Zhejiang Provice (2015c12001) Ntọala Science Science nke China (42007354).
Esemokwu nke Mmasị
Ndị dere akwụkwọ na-ekwupụta na ha enweghị esemokwu nke mmasị.
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Copyright: © 2022 nke ode akwụkwọ (s). Akwụkwọ ikike agụmakwụkwọ kachasị masị, Fayetteville, Ga. Edemede a bu ederede oghe n'okpuru ihe eji eme ihe eji eme ihe (CC site na Creative Creative Common.//creaveNoMommons.org/by/4.0/.
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Banyere edemede a
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